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Western blot(SDS PAGE) analysis of extracts from U-937 cells.Using FCGR2A / CD32a Mouse mAb IgG [K667] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :FCGR2A,CD32,FCG2,FCGR2A1,IGFR2,FCG2A_HUMAN,Low affinity immunoglobulin gamma Fc region receptor II-a
UniProtID :P12318
MASS(da) :35,001
MW(kDa) :42kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human FCGR2A / CD32a.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :U-937 cells
Function : Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens..
Tissue specificity :Found on monocytes, neutrophils and eosinophil platelets.
Subcellular locationi :Cell membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.