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Western blot(SDS PAGE) analysis of extracts from Hepg2 cells.Using Fetuin-A Mouse mAb IgG [L2RP] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :AHSG,FETUA,PRO2743,FETUA_HUMAN,Alpha-2-HS-glycoprotein [Cleaved into: Alpha-2-HS-glycoprotein chain A,Alpha-2-HS-glycoprotein chain B]
UniProtID :P02765
MASS(da) :39,341
MW(kDa) :58kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Fetuin-A.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Hepg2 cells
Function : Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions.
Tissue specificity :Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins.
Subcellular locationi :Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.