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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using FUCA1 Mouse mAb IgG [9I2T] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :FUCA1,Nbla10230,FUCO_HUMAN,Tissue alpha-L-fucosidase
UniProtID :P04066
MASS(da) :53,689
MW(kDa) :56kda
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human FUCA1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins..
Subcellular locationi :Lysosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.