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Western blot(SDS PAGE) analysis of extracts from mouse cerebellum tissue.Using GAD1 Mouse mAb IgG [FSX3] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :GAD1,GAD,GAD67,DCE1_HUMAN,Glutamate decarboxylase 1
UniProtID :Q99259
MASS(da) :66,897
MW(kDa) :67kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human GAD1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :mouse cerebellum tissue
Function : Catalyzes the synthesis of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) with pyridoxal 5'-phosphate as cofactor.., [Isoform 3]: Enzymatically inactive as glutamate decarboxylase..
Tissue specificity :[Isoform 1]: Expressed in brain..,[Isoform 3]: Expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.