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Western blot(SDS PAGE) analysis of extracts from U-251 cells.Using GDI1 Mouse mAb IgG [M9P2] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :GDI1,GDIL,OPHN2,RABGDIA,XAP4,GDIA_HUMAN,Rab GDP dissociation inhibitor alpha
UniProtID :P31150
MASS(da) :50,583
MW(kDa) :55 kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human GDI1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :U-251 cells
Function : Regulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Promotes the dissociation of GDP-bound Rab proteins from the membrane and inhibits their activation. Promotes the dissociation of RAB1A, RAB3A, RAB5A and RAB10 from membranes..
Tissue specificity :Brain,predominant in neural and sensory tissues..
Subcellular locationi :Cytoplasm. Golgi apparatus, trans-Golgi network.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.