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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using GDI2 Mouse mAb IgG [486V] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :GDI2,RABGDIB,GDIB_HUMAN,Rab GDP dissociation inhibitor beta
UniProtID :P50395
MASS(da) :50,663
MW(kDa) :46kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human GDI2.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : GDP-dissociation inhibitor preventing the GDP to GTP exchange of most Rab proteins. By keeping these small GTPases in their inactive GDP-bound form regulates intracellular membrane trafficking (PubMed:25860027). Negatively regulates protein transport to the cilium and ciliogenesis through the inhibition of RAB8A (PubMed:25860027)..
Tissue specificity :Ubiquitous..
Subcellular locationi :Cytoplasm. Membrane,Peripheral membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.