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GDI2 Mouse mAb[486V]Cat NO.: A53338

Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using GDI2 Mouse mAb IgG [486V] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :GDI2,RABGDIB,GDIB_HUMAN,Rab GDP dissociation inhibitor beta

UniProtID :P50395

MASS(da) :50,663

MW(kDa) :46kDa

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human GDI2.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells

Function : GDP-dissociation inhibitor preventing the GDP to GTP exchange of most Rab proteins. By keeping these small GTPases in their inactive GDP-bound form regulates intracellular membrane trafficking (PubMed:25860027). Negatively regulates protein transport to the cilium and ciliogenesis through the inhibition of RAB8A (PubMed:25860027)..

Tissue specificity :Ubiquitous..

Subcellular locationi :Cytoplasm. Membrane,Peripheral membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.