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Western blot(SDS PAGE) analysis of extracts from NIH/3T3 cells.Using HSP47 Mouse mAb IgG [775G] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SERPINH1,CBP1,CBP2,HSP47,SERPINH2,PIG14,SERPH_HUMAN,Serpin H1
UniProtID :P50454
MASS(da) :46,441
MW(kDa) :46kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human HSP47.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :NIH/3T3 cells
Function : Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
Subcellular locationi :Endoplasmic reticulum lumen.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.