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HSP47 Mouse mAb[775G]Cat NO.: A13597

Western blot(SDS PAGE) analysis of extracts from NIH/3T3 cells.Using HSP47 Mouse mAb IgG [775G] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :SERPINH1,CBP1,CBP2,HSP47,SERPINH2,PIG14,SERPH_HUMAN,Serpin H1

UniProtID :P50454

MASS(da) :46,441

MW(kDa) :46kDa

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human HSP47.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :NIH/3T3 cells

Function : Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.

Subcellular locationi :Endoplasmic reticulum lumen.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.