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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using HSPH1 Mouse mAb IgG [44AF] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :HSPH1,HSP105,HSP110,KIAA0201,HS105_HUMAN,Heat shock protein 105 kDa
UniProtID :Q92598
MASS(da) :96,865
MW(kDa) :110kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human HSPH1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Acts as a nucleotide-exchange factor (NEF) for chaperone proteins HSPA1A and HSPA1B, promoting the release of ADP from HSPA1A/B thereby triggering client/substrate protein release (PubMed:24318877). Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities (By similarity)..
Tissue specificity :Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells..
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.