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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using LAMC1 Mouse mAb IgG [TU83] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :LAMC1,LAMB2,LAMC1_HUMAN,Laminin subunit gamma-1
UniProtID :P11047
MASS(da) :177,603
MW(kDa) :200kda
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human LAMC1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Tissue specificity :Found in the basement membranes (major component).
Subcellular locationi :Secreted, extracellular space, extracellular matrix, basement membrane.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.