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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using MAT2B Mouse mAb IgG [I5JL] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MAT2B,TGR,MSTP045,Nbla02999,UNQ2435/PRO4995,MAT2B_HUMAN,Methionine adenosyltransferase 2 subunit beta
UniProtID :Q9NZL9
MASS(da) :37,552
MW(kDa) :38kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human MAT2B.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Regulatory subunit of S-adenosylmethionine synthetase 2, an enzyme that catalyzes the formation of S-adenosylmethionine from methionine and ATP. Regulates MAT2A catalytic activity by changing its kinetic properties, increasing its affinity for L-methionine (PubMed:10644686, PubMed:23189196, PubMed:25075345). Can bind NADP (in vitro) (PubMed:23189196, PubMed:23425511)..
Tissue specificity :Widely expressed..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.