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MCL1 Mouse mAb[E675]Cat NO.: A39934

Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using MCL1 Mouse mAb IgG [E675] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :MCL1,BCL2L3,MCL1_HUMAN,Induced myeloid leukemia cell differentiation protein Mcl-1

UniProtID :Q07820

MASS(da) :37,337

MW(kDa) :37kDa

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human MCL1.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells

Function : Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis..

Subcellular locationi :Membrane,Single-pass membrane protein. Cytoplasm. Mitochondrion. Nucleus, nucleoplasm.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.