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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using MPG Mouse mAb IgG [60IC] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MPG,AAG,ANPG,MID1,3MG_HUMAN,DNA-3-methyladenine glycosylase
UniProtID :P29372
MASS(da) :32,869
MW(kDa) :35kda
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human MPG.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.
Subcellular locationi :Cytoplasm. Mitochondrion matrix, mitochondrion nucleoid. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.