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Western blot(SDS PAGE) analysis of extracts from 293T cells treated with CCCP( 100 μM 2 hr) and hBeclin-1.Using P-Beclin-1 (S30) Rabbit mAb [R2LH] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :BECN1,GT197,BECN1_HUMAN,Beclin-1 [Cleaved into: Beclin-1-C 35 kDa,Beclin-1-C 37 kDa]
UniProtID :Q14457
MASS(da) :51,896
MW(kDa) :60 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human Phospho-Beclin-1 (Ser30)
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :293T cells treated with CCCP( 100 μM 2 hr) and hBeclin-1
Function : Plays a central role in autophagy (PubMed:18570871, PubMed:21358617, PubMed:23184933, PubMed:23974797, PubMed:28445460). Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate,different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123, PubMed:20208530, PubMed:23974797, PubMed:26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). Protects against infection by a neurovirulent strain of Sindbis virus (PubMed:9765397). May play a role in antiviral host defense.., Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis,it induces the mitochondrial translocation of BAX and the release of proapoptotic factors..
Tissue specificity :Ubiquitous.
Subcellular locationi :Cytoplasm. Golgi apparatus, trans-Golgi network membrane,Peripheral membrane protein. Endosome membrane,Peripheral membrane protein. Endoplasmic reticulum membrane,Peripheral membrane protein. Mitochondrion membrane,Peripheral membrane protein. Endosome. Cytoplasmic vesicle, autophagosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.