MUS81 Mouse mAb[6YBG]Cat NO.: A99896

Protein names ：MUS81,MUS81_HUMAN,Crossover junction endonuclease MUS81

UniProtID ：Q96NY9

MASS(da) ：61,173

MW(kDa) ：72kda

Form ：Liquid

Purification ：Protein A purification

Host ：Mouse

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide of human MUS81.

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：MCF-7 cells

Function ： Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. Plays an essential role in mitosis for the processing of stalled or collapsed replication forks (PubMed:28575661)..

Tissue specificity ：Widely expressed.

Subcellular locationi ：Nucleus, nucleolus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.