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Western blot(SDS PAGE) analysis of extracts from A549 cells.Using NHE1 Mouse mAb IgG [DVGS] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SLC9A1,APNH1,NHE1,SL9A1_HUMAN,Sodium/hydrogen exchanger 1 /H antiporter, amiloride-sensitive) /H exchanger 1)
UniProtID :P19634
MASS(da) :90,763
MW(kDa) :110kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human NHE1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A549 cells
Function : Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction..
Tissue specificity :Kidney and intestine.
Subcellular locationi :Membrane,Multi-pass membrane protein. Endoplasmic reticulum membrane,Multi-pass membrane protein. Cell membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.