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Western blot(SDS PAGE) analysis of extracts from MOLT-4 cells.Using Brd2 Rabbit mAb [M267] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :BRD2,KIAA9001,RING3,BRD2_HUMAN,Bromodomain-containing protein 2
UniProtID :P25440
MASS(da) :88,061
MW(kDa) :110 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human Brd2
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MOLT-4 cells
Function : May play a role in spermatogenesis or folliculogenesis (By similarity). Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling. Regulates transcription of the CCND1 gene. Plays a role in nucleosome assembly..
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.