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Western blot(SDS PAGE) analysis of extracts from MCF-7 cells.Using PEX1 Mouse mAb IgG [T9HT] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PEX1,PEX1_HUMAN,Peroxisome biogenesis factor 1
UniProtID :O43933
MASS(da) :142,867
MW(kDa) :143kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human PEX1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MCF-7 cells
Function : Required for stability of PEX5 and protein import into the peroxisome matrix. Anchored by PEX26 to peroxisome membranes, possibly to form heteromeric AAA ATPase complexes required for the import of proteins into peroxisomes.
Subcellular locationi :Cytoplasm. Peroxisome membrane.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.