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Western blot(SDS PAGE) analysis of extracts from LNCaP cells.Using PGD Mouse mAb IgG [03IF] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PGD,PGDH,6PGD_HUMAN,6-phosphogluconate dehydrogenase, decarboxylating
UniProtID :P52209
MASS(da) :53,140
MW(kDa) :53kDa,45kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human PGD.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :LNCaP cells
Function : Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH..
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.