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Western blot(SDS PAGE) analysis of extracts from HEK-293 cells.Using RAB18 Mouse mAb IgG [6V6Y] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :RAB18,RAB18_HUMAN,Ras-related protein Rab-18
UniProtID :Q9NP72
MASS(da) :22,977
MW(kDa) :23kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human RAB18.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HEK-293 cells
Function : Required for the localization of ZFYVE1 to lipid droplets and for its function in mediating the formation of endoplasmic reticulum-lipid droplets (ER-LD) contacts (PubMed:30970241). Plays a role in apical endocytosis/recycling (By similarity). Plays a key role in eye and brain development and neurodegeneration (PubMed:21473985)..
Tissue specificity :Ubiquitous.
Subcellular locationi :Apical cell membrane. Lipid droplet.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.