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Western blot(SDS PAGE) analysis of extracts from HEK-293 cells.Using RHOQ/TC10 Mouse mAb IgG [IZ30] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :RHOQ,ARHQ,RASL7A,TC10,RHOQ_HUMAN,Rho-related GTP-binding protein RhoQ
UniProtID :P17081
MASS(da) :22,659
MW(kDa) :23kda
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human RHOQ/TC10.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HEK-293 cells
Function : Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. May play a role in CFTR trafficking to the plasma membrane. Causes the formation of thin, actin-rich surface projections called filopodia..
Subcellular locationi :Cytoplasm. Cell membrane,Lipid-anchor.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.