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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using SLC9A9 Mouse mAb IgG [86SD] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SLC9A9,NHE9,Nbla00118,SL9A9_HUMAN,Sodium/hydrogen exchanger 9 /H exchanger 9)
UniProtID :Q8IVB4
MASS(da) :72,565
MW(kDa) :73kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human SLC9A9.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : May act in electroneutral exchange of protons for Na(+) across membranes. Involved in the effusion of Golgi luminal H(+) in exchange for cytosolic cations. Involved in organelle ion homeostasis by contributing to the maintenance of the unique acidic pH values of the Golgi and post-Golgi compartments in the cell..
Tissue specificity :Ubiquitously expressed in all tissues tested. Expressed at highest levels in heart and skeletal muscle, followed by placenta, kidney, and liver. Expressed in the brain, in the medulla and spinal cord..
Subcellular locationi :Late endosome membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.