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Western blot(SDS PAGE) analysis of extracts from fetal human brain tissue.Using SNAP25 Mouse mAb IgG [00V2] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SNAP25,SNAP,SNP25_HUMAN,Synaptosomal-associated protein 25
UniProtID :P60880
MASS(da) :23,315
MW(kDa) :25kda
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human SNAP25.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :fetal human brain tissue
Function : t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells..
Tissue specificity :Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
Subcellular locationi :Cytoplasm, perinuclear region. Cell membrane,Lipid-anchor. Cell junction, synapse, synaptosome. Photoreceptor inner segment.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.