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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using Talin-1 Mouse mAb IgG [T9W1] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TLN1,KIAA1027,TLN,TLN1_HUMAN,Talin-1
UniProtID :Q9Y490
MASS(da) :269,767
MW(kDa) :270kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Talin-1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts (By similarity)..
Subcellular locationi :Cell projection, ruffle membrane,Peripheral membrane protein,Cytoplasmic side. Cytoplasm, cytoskeleton. Cell surface. Cell junction, focal adhesion.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.