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Western blot(SDS PAGE) analysis of extracts from LNCaP cells.Using TOMM40 Mouse mAb IgG [J4L4] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TOMM40,C19orf1,PEREC1,TOM40,TOM40_HUMAN,Mitochondrial import receptor subunit TOM40 homolog
UniProtID :O96008
MASS(da) :37,893
MW(kDa) :38kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human TOMM40.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :LNCaP cells
Function : Channel-forming protein essential for import of protein precursors into mitochondria (PubMed:15644312, PubMed:31206022). Plays a role in the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) by forming a complex with BCAP31 and mediating the translocation of Complex I components from the cytosol to the mitochondria (PubMed:31206022)..
Subcellular locationi :Mitochondrion outer membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.