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Western blot(SDS PAGE) analysis of extracts from human plasma.Using Transferrin Mouse mAb IgG [NCLA] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TF,PRO1400,TRFE_HUMAN,Serotransferrin
UniProtID :P02787
MASS(da) :77,064
MW(kDa) :77kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Transferrin.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :human plasma
Function : Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation., (Microbial infection) Serves as an iron source for Neisseria species, which capture the protein and extract its iron for their own use..
Tissue specificity :Expressed by the liver and secreted in plasma.
Subcellular locationi :Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.