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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using TRAPA/SSR1 Mouse mAb IgG [4SZ5] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SSR1,TRAPA,PSEC0262,SSRA_HUMAN,Translocon-associated protein subunit alpha
UniProtID :P43307
MASS(da) :32,235
MW(kDa) :34kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human TRAPA/SSR1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. May be involved in the recycling of the translocation apparatus after completion of the translocation process or may function as a membrane-bound chaperone facilitating folding of translocated proteins.
Subcellular locationi :Endoplasmic reticulum membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.