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Western blot(SDS PAGE) analysis of extracts from rat brain tissue.Using TRIM2 Mouse mAb IgG [7KH8] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TRIM2,KIAA0517,RNF86,TRIM2_HUMAN,Tripartite motif-containing protein 2
UniProtID :Q9C040
MASS(da) :81,530
MW(kDa) :85kda
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human TRIM2.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :rat brain tissue
Function : UBE2D1-dependent E3 ubiquitin-protein ligase that mediates the ubiquitination of NEFL and of phosphorylated BCL2L11. Plays a neuroprotective function. May play a role in neuronal rapid ischemic tolerance..
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.