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Western blot(SDS PAGE) analysis of extracts from rat liver tissue.Using TTPA Mouse mAb IgG [DN07] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TTPA,TPP1,TTPA_HUMAN,Alpha-tocopherol transfer protein
UniProtID :P49638
MASS(da) :31,750
MW(kDa) :32kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human TTPA.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :rat liver tissue
Function : Binds alpha-tocopherol, enhances its transfer between separate membranes, and stimulates its release from liver cells (PubMed:7887897). Binds both phosphatidylinositol 3,4-bisphosphate and phosphatidylinositol 4,5-bisphosphate,the resulting conformation change is important for the release of the bound alpha-tocopherol (By similarity)..
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.