支持:13376277882 | tech@naturebios.com

UBE1 Mouse mAb[536M]Cat NO.: A69656

Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using UBE1 Mouse mAb IgG [536M] at dilution of 1:1000 incubated at 4℃ over night.

  • SizePriceIn stock
  • 50ul1280咨询客服
  • 100ul2380咨询客服
订单 Datasheet

Product information

Protein names :UBA1,A1S9T,UBE1,UBA1_HUMAN,Ubiquitin-like modifier-activating enzyme 1

UniProtID :P22314

MASS(da) :117,849

MW(kDa) :118kda

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human UBE1.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells

Function : Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system (PubMed:1606621, PubMed:1447181, PubMed:33108101). Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed:1447181). Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (PubMed:22456334)..

Tissue specificity :Detected in erythrocytes (at protein level). Ubiquitous..

Subcellular locationi :Cytoplasm. Mitochondrion. Nucleus.,[Isoform 1]: Nucleus.,[Isoform 2]: Cytoplasm.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.