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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using UBE1 Mouse mAb IgG [536M] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :UBA1,A1S9T,UBE1,UBA1_HUMAN,Ubiquitin-like modifier-activating enzyme 1
UniProtID :P22314
MASS(da) :117,849
MW(kDa) :118kda
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human UBE1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system (PubMed:1606621, PubMed:1447181, PubMed:33108101). Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed:1447181). Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (PubMed:22456334)..
Tissue specificity :Detected in erythrocytes (at protein level). Ubiquitous..
Subcellular locationi :Cytoplasm. Mitochondrion. Nucleus.,[Isoform 1]: Nucleus.,[Isoform 2]: Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.