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Western blot(SDS PAGE) analysis of extracts from A549 cells.Using VAPB Mouse mAb IgG [1JXH] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :VAPB,UNQ484/PRO983,VAPB_HUMAN,Vesicle-associated membrane protein-associated protein B/C
UniProtID :O95292
MASS(da) :27,228
MW(kDa) :30kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human VAPB.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A549 cells
Function : Participates in the endoplasmic reticulum unfolded protein response (UPR) by inducing ERN1/IRE1 activity. Involved in cellular calcium homeostasis regulation..
Tissue specificity :Ubiquitous. Isoform 1 predominates.
Subcellular locationi :Endoplasmic reticulum membrane,Single-pass type IV membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.