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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using VAT1 Mouse mAb IgG [V45Q] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :VAT1,VAT1_HUMAN,Synaptic vesicle membrane protein VAT-1 homolog
UniProtID :Q99536
MASS(da) :41,920
MW(kDa) :42kda
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human VAT1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Possesses ATPase activity (By similarity). Plays a part in calcium-regulated keratinocyte activation in epidermal repair mechanisms. Has no effect on cell proliferation. Negatively regulates mitochondrial fusion in cooperation with mitofusin proteins (MFN1-2)..
Tissue specificity :Expressed in brain. Also expressed in glioblastoma cells..
Subcellular locationi :Cytoplasm. Mitochondrion outer membrane,Peripheral membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.