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Western blot(SDS PAGE) analysis of extracts from A549 cells.Using AHCYL2 Mouse mAb IgG [Q5G2] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :AHCYL2,KIAA0828,SAHH3_HUMAN,Adenosylhomocysteinase 3 Rs binding protein released with IP 2)
UniProtID :Q96HN2
MASS(da) :66,721
MW(kDa) :67and57kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human AHCYL2.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A549 cells
Function : May regulate the electrogenic sodium/bicarbonate cotransporter SLC4A4 activity and Mg(2+)-sensitivity. On the contrary of its homolog AHCYL1, does not regulate ITPR1 sensitivity to inositol 1,4,5-trisphosphate (PubMed:19220705)..
Subcellular locationi :Cytoplasm. Microsome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.