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Western blot(SDS PAGE) analysis of extracts from Jurkat cells.Using AP5Z1/SPG48 Mouse mAb IgG [318C] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :AP5Z1,KIAA0415,SPG48,AP5Z1_HUMAN,AP-5 complex subunit zeta-1
UniProtID :O43299
MASS(da) :88,605
MW(kDa) :88kDa,160kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human AP5Z1/SPG48.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Jurkat cells
Function : As part of AP-5, a probable fifth adaptor protein complex it may be involved in endosomal transport. According to PubMed:20613862 it is a putative helicase required for efficient homologous recombination DNA double-strand break repair..
Subcellular locationi :Cytoplasm. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.