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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using eIF4G Rabbit mAb [V39R] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :EIF4G1,EIF4F,EIF4G,EIF4GI,IF4G1_HUMAN,Eukaryotic translation initiation factor 4 gamma 1
UniProtID :Q04637
MASS(da) :175,491
MW(kDa) :220 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human eIF4G
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. As a member of the eIF4F complex, required for endoplasmic reticulum stress-induced ATF4 mRNA translation (PubMed:29062139)..
Subcellular locationi :Cytoplasm, Stress granule.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.