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Western blot(SDS PAGE) analysis of extracts from MCF-7 cells.Using CD82 Mouse mAb IgG [ZIOB] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CD82,KAI1,SAR2,ST6,TSPAN27,CD82_HUMAN,CD82 antigen
UniProtID :P27701
MASS(da) :29,626
MW(kDa) :48kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human CD82.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MCF-7 cells
Function : Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.
Tissue specificity :Lymphoid specific.
Subcellular locationi :Cell membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.