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Western blot(SDS PAGE) analysis of extracts from mouse brain tissue.Using CNTROB Mouse mAb IgG [04IB] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CNTROB,LIP8,PP1221,CNTRB_HUMAN,Centrobin
UniProtID :Q8N137
MASS(da) :101,253
MW(kDa) :97kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human CNTROB.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :mouse brain tissue
Function : Required for centriole duplication. Inhibition of centriole duplication leading to defects in cytokinesis..
Tissue specificity :Widely expressed (at protein level). Highly expressed in testis. Also expressed in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes..
Subcellular locationi :Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.