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Western blot(SDS PAGE) analysis of extracts from HEK-293 cells.Using EIF1 Mouse mAb IgG [J297] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :EIF1,SUI1,EIF1_HUMAN,Eukaryotic translation initiation factor 1
UniProtID :P41567
MASS(da) :12,732
MW(kDa) :13kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human EIF1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HEK-293 cells
Function : Necessary for scanning and involved in initiation site selection. Promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.