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Western blot(SDS PAGE) analysis of extracts from HUVEC cells.Using EMCN Mouse mAb IgG [OW31] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :EMCN,EMCN2,MUC14,MUCEN_HUMAN,Endomucin
UniProtID :Q9ULC0
MASS(da) :27,452
MW(kDa) :120kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human EMCN.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HUVEC cells
Function : Endothelial sialomucin, also called endomucin or mucin-like sialoglycoprotein, which interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix.
Tissue specificity :Expressed in heart, kidney and lung..
Subcellular locationi :[Isoform 1]: Cell membrane,Single-pass type I membrane protein. Membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.