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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using ESYT1 Mouse mAb IgG [7H80] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ESYT1,FAM62A,KIAA0747,MBC2,ESYT1_HUMAN,Extended synaptotagmin-1
UniProtID :Q9BSJ8
MASS(da) :122,856
MW(kDa) :123kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human ESYT1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Binds glycerophospholipids in a barrel-like domain and may play a role in cellular lipid transport (By similarity). Binds calcium (via the C2 domains) and translocates to sites of contact between the endoplasmic reticulum and the cell membrane in response to increased cytosolic calcium levels. Helps tether the endoplasmic reticulum to the cell membrane and promotes the formation of appositions between the endoplasmic reticulum and the cell membrane..
Tissue specificity :Widely expressed..
Subcellular locationi :Endoplasmic reticulum membrane,Multi-pass membrane protein. Cell membrane,Peripheral membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.