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Western blot(SDS PAGE) analysis of extracts from human placenta tissue.Using Factor XIIIa Mouse mAb IgG [5Y3E] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :F13A1,F13A,F13A_HUMAN,Coagulation factor XIII A chain
UniProtID :P00488
MASS(da) :83,267
MW(kDa) :80kda
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Factor XIIIa.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :human placenta tissue
Function : Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin..
Subcellular locationi :Cytoplasm. Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.