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Western blot(SDS PAGE) analysis of extracts from MCF-7 cells.Using GATA3 Mouse mAb IgG [8LTV] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :GATA3,GATA3_HUMAN,Trans-acting T-cell-specific transcription factor GATA-3
UniProtID :P23771
MASS(da) :47,916
MW(kDa) :50kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human GATA3.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MCF-7 cells
Function : Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. Positively regulates ASB2 expression (By similarity)..
Tissue specificity :T-cells and endothelial cells.
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.