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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using GNB3 Mouse mAb IgG [4IP7] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :GNB3,GBB3_HUMAN,Guanine nucleotide-binding protein G/G/G subunit beta-3
UniProtID :P16520
MASS(da) :37,221
MW(kDa) :37kda
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human GNB3.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.