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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using Maspin Mouse mAb IgG [25EP] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SERPINB5,PI5,SPB5_HUMAN,Serpin B5
UniProtID :P36952
MASS(da) :42,100
MW(kDa) :40kda
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Maspin.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
Tissue specificity :Normal mammary epithelial cells.
Subcellular locationi :Secreted, extracellular space.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.