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Maspin Mouse mAb[25EP]Cat NO.: A89065

Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using Maspin Mouse mAb IgG [25EP] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :SERPINB5,PI5,SPB5_HUMAN,Serpin B5

UniProtID :P36952

MASS(da) :42,100

MW(kDa) :40kda

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Maspin.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HepG2 cells

Function : Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

Tissue specificity :Normal mammary epithelial cells.

Subcellular locationi :Secreted, extracellular space.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.