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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using MBIP Mouse mAb IgG [63O8] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MBIP,BM-015,MBIP1_HUMAN,MAP3K12-binding inhibitory protein 1
UniProtID :Q9NS73
MASS(da) :39,281
MW(kDa) :39kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human MBIP.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Inhibits the MAP3K12 activity to induce the activation of the JNK/SAPK pathway. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4..
Tissue specificity :Ubiquitous. High expression seen in the heart and lung.
Subcellular locationi :Nucleus. Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.