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Western blot(SDS PAGE) analysis of extracts from Jurkat cells.Using NOB1 Mouse mAb IgG [OXJ1] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :NOB1,ART4,NOB1P,PSMD8BP1,MSTP158,NOB1_HUMAN,RNA-binding protein NOB1
UniProtID :Q9ULX3
MASS(da) :46,675
MW(kDa) :47kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human NOB1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Jurkat cells
Function : May play a role in mRNA degradation (Probable). Endonuclease required for processing of 20S pre-rRNA precursor and biogenesis of 40S ribosomal subunits (By similarity)..
Tissue specificity :Detected in liver, lung, placenta, endothelial cells and spleen..
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.