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PHOX2B Mouse mAb[1108]Cat NO.: A47583

Western blot(SDS PAGE) analysis of extracts from Neuro-2a cells.Using PHOX2B Mouse mAb IgG [1108] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :PHOX2B,PMX2B,PHX2B_HUMAN,Paired mesoderm homeobox protein 2B

UniProtID :Q99453

MASS(da) :31,621

MW(kDa) :32kda

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human PHOX2B.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Neuro-2a cells

Function : Involved in the development of several major noradrenergic neuron populations, including the locus coeruleus. Transcription factor which could determine a neurotransmitter phenotype in vertebrates. Enhances second-messenger-mediated activation of the dopamine beta-hydrolase and c-fos promoters, and of several enhancers including cAMP-response element and serum-response element.

Tissue specificity :Expressed in neuroblastoma, brain and adrenal gland.

Subcellular locationi :Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.