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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using RARS Mouse mAb IgG [MH6I] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :RARS1,RARS,SYRC_HUMAN,Arginine--tRNA ligase, cytoplasmic
UniProtID :P54136
MASS(da) :75,379
MW(kDa) :67kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human RARS.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis (PubMed:25288775). Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1 (PubMed:17443684)..
Subcellular locationi :Cytoplasm. Cytoplasm, cytosol.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.