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Western blot(SDS PAGE) analysis of extracts from MCF-7 cells.Using SMYD4 Mouse mAb IgG [6099] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SMYD4,KIAA1936,SMYD4_HUMAN,SET and MYND domain-containing protein 4
UniProtID :Q8IYR2
MASS(da) :89,225
MW(kDa) :89kda
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human SMYD4.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MCF-7 cells
Function : Plays a critical role in cardiac development (PubMed:30110327). Acts as a key epigenetic regulator of gene expression during cardiac development via its dual activities as a methyltransferase and negative regulator of HDAC1 (By similarity)..
Subcellular locationi :Nucleus. Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.