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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using Thrombospondin 1 Mouse mAb IgG [MF63] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :THBS1,TSP,TSP1,TSP1_HUMAN,Thrombospondin-1
UniProtID :P07996
MASS(da) :129,383
MW(kDa) :160kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Thrombospondin 1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity). Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors (By similarity)..
Subcellular locationi :Secreted. Cell surface. Secreted, extracellular space, extracellular matrix. Endoplasmic reticulum. Sarcoplasmic reticulum.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.