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Western blot(SDS PAGE) analysis of extracts from Mouse eye Tissue lysate.Using CRYAA Rabbit mAb [373I] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Cryaa,Crya1,CRYAA_MOUSE,Alpha-crystallin A chain
UniProtID :P24622
MASS(da) :22,489
MW(kDa) :18KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit monoclonal IgG
Isotype :IgG
sensitivity :Endogenous
Reactivity :Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Mouse CRYAA.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Mouse eye Tissue lysate
Function : Contributes to the transparency and refractive index of the lens. Acts as a chaperone, preventing aggregation of various proteins under a wide range of stress conditions. Required for the correct formation of lens intermediate filaments as part of a complex composed of BFSP1, BFSP2 and CRYAA..
Subcellular locationi :Cytoplasm. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.